Journal: PLoS ONE

Article Title: Concave Pit-Containing Scaffold Surfaces Improve Stem Cell-Derived Osteoblast Performance and Lead to Significant Bone Tissue Formation

doi: 10.1371/journal.pone.0000496

Figure Lengend Snippet: The panel shows positivity for Collagen I ( A ) FITC (green) (Calibration Bar = 10 µm), BAP [Bone Alkaline Phosphatase] ( B ) FITC (green) (Calibration Bar = 5 µm), OC [Osteocalcin] ( C ) PE (red) (Calibration Bar = 7 µm), ON [Osteonectin] ( D ) FITC(green) (Calibration Bar = 7 µm) and BSP [Bone Sialoprotein] ( E ) PE (red) (Calibration Bar = 3 µm). The same analysis confirming the presence of a mineralized extra cellular matrix on smooth texturing. The panel shows positivity for Collagen I ( F ) FITC (green) (Calibration Bar = 5 µm), BAP ( G ) FITC (green) (Calibration Bar = 7 µm), OC ( H ) PE (red) (Calibration Bar = 3 µm), ON ( I ) FITC (green) (Calibration Bar = 7 µm) and BSP ( J ) PE (red) (Calibration Bar = 3 µm). Nuclear staining is obtained with DAPI (blue).

Article Snippet: Mouse anti-human monoclonal antibodies were the following: bone alkaline phosphatase (BAP), osteonectin (US Biological, Swampscott, MA); anti-osteocalcin, anti-osteonectin, anti-BSP and anti- Collagen I (Santa Cruz, CA) were goat anti-human.

Techniques: Staining